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UID:pretalx-soil-health-now-2025-SADJJP@pretalx.earthmonitor.org
DTSTART:20250408T180000Z
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DESCRIPTION:The comprehensive characterization of soil microbiomes is pivot
 al for understanding ecosystem functions and managing soil health. The aim
  of the study is to develop a standardized metabarcoding pipeline utilizin
 g universal genetic markers\, such as the 16S rRNA and ITS and it is also 
 possible for specific markers that target genes associated with critical m
 etabolic functions. Sequencing is conducted using the MinION platform by O
 xford Nanopore Technologies\, enabling real-time data acquisition and anal
 ysis.\n\nA key component of this pipeline is the creation of a qualified d
 atabase comprising accurately annotated sequences derived from standardize
 d metabarcoding protocols. This database\, enhanced by the custom analysis
  tools available through the EPI2ME labs platform\, serves as a foundation
 al tool for ensuring reproducibility and comparability across studies\, ad
 dressing the current challenge of data heterogeneity due to varied experim
 ental and analytical methodologies. This database not only enhances the ta
 xonomic resolution but can also potentially enrich the functional profilin
 g of soil microbiomes.\n\nThe research incorporates two distinct series of
  soil samples: one from wheat fields cultivated in Sicily and another from
  greenhouse-grown tomatoes. These diverse agricultural settings provide tw
 o case studies with a broad spectrum of microbial communities\, to test th
 e robustness and applicability of the metabarcoding pipeline.\n\nThe stand
 ardized data from this qualified database can be exploited to train artifi
 cial intelligence (AI) models\, aiming to identify predictive signatures o
 f soil health and potential for reclamation from pollutants. By applying m
 achine learning techniques to metabarcoding data\, this approach promises 
 to uncover novel correlations between microbial communities and soil condi
 tions\, potentially leading to innovative strategies for soil management a
 nd rehabilitation.\n\nIn summary\, the establishment of a standardized met
 abarcoding pipeline and a qualified database on the MinION platform\, enri
 ched with data from diverse agricultural soils\, represents a significant 
 advancement in soil microbiology. This framework not only enhances our und
 erstanding of microbial diversity and function but also offers the possibi
 lity to apply AI technologies to predict and improve soil health on a glob
 al scale.
DTSTAMP:20260807T115230Z
LOCATION:W - Invite
SUMMARY:Standardized metabarcoding pipeline for soil microbiome analysis on
  MinION platform. - Fabio Fracchetti
URL:https://pretalx.earthmonitor.org/soil-health-now-2025/talk/SADJJP/
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