Soils are increasingly rediscovered as a vital resource that underpins many natural and societal services. Over more than half a century, agricultural mechanization and a singular focus on plant production, supported by chemical fertilizers, have led to widespread soil degradation. This reductionistic perspective has relied on soil observations focused on physico-chemical properties; properties that can be boosted by chemical additions but ignore the biological and ecological status and functions of the soil. Recognizing the importance of natural soil processes, which have evolved and been fine-tuned over billions of years, a new set of indicators for describing soil health beyond the physico-chemical properties is required. These indicators should preferably be observable and analyzable by farmers, advisors, extension workers and other citizen scientists. Methods that directly or indirectly capture the biological and ecological functions include, for instance i) environmental DNA (eDNA) metabarcoding to characterize the diversity and composition of soil microbial communities, ii) activity rates of key enzymes involved in the main biogeochemical cycles, iii) the ratio of soil fungi to bacteria, an indicator of the extent of disturbance in soil ecosystems, iv) aggregate stability, which is important for soil erosion resistance, and water and nutrient holding capacity, and v) water infiltration capacity as a key measure of the soil water absorption, holding and release potentials. While eDNA requires specialist laboratories and databases, the other methods are currently available for “Do-It-Yourself” (DIY) testing. In this study, as part of the EU-funded project AI4SoilHealth (https://ai4soilhealth.eu) we sampled soils in Greece, Sweden, Finland, Croatia and Denmark. We applied the outlined methods alongside traditional wet chemistry analysis of properties such as carbon, pH and electrical conductivity, and the particle size distribution. These properties were also estimated by leveraging their correlations with diffuse reflectance Near InfraRed (NIR) spectra and applying machine learning models. We are testing both the robustness of the novel methods and their interdependence with more traditional physico-chemical properties and soil spectroscopy. We hypothesize that there is a significant positive correlation between novel indicators (e.g. eDNA richness is correlated to enzymatic activity, which is correlated to aggregate stability, which in turn is correlated to infiltration capacity) and that high scores of the biological and ecological properties are correlated with, for instance, soil carbon content. This study explores the potential of these novel methods for more holistic understanding of soil health.